Molecular Biological Investigations of Endemic Nephropathy

Abstract

Today, the investigation of different human diseases cannot be successful without involving of this disease molecular aspect. It means modem medicine and molecular biology is fortunately irreversible connected disciplines that together provide disease investigation on the level of informatics macromolecules (DNA and RNA). It is now possible to detect causative agent of some disease4 very fast with sensitivity and specificity almost 100%. Molecular biology provides important information about many human diseases such as Diabetes mellitus-1, Familial adenomatous polyposis, Cystic fibrosis and in the same time is crucial in prenatal diagnostics and transplantation medicine. Molecular aspect of endemic nephropathy investigation, of course, provides also confirmation of wide accepted hypothesis about genetic predispositions of humans to this disease. BEN (Balkan endemic nephropathy) is disease of not yet known etiology. By using of several molecular methods, it is determined that 3q21.3-3q27.3 region of human 3 chromosome is connected with BEN. The investigation of polymorphic microsatellite DNA sequences of this region will be useful in solving of BEN genetic background. But priority surely will be finding of main aethiological factors included in making of BEN specific genetic disorders. Only multidisciplinary approach in solving of this disease will be successful, in which molecular biology will play very important role.