Using of Broadly Reactive Primer Pair in RT-PCR Detection of Small Round-Structured Viruses (SRSVS)

Authors

  • Đemo Subašić Institute of microbiology, immunnology and parasitology, Clinical center University of Sarajevo, Bosnia and Herzegovina
  • Kemal Šerić Institute of microbiology, immunnology and parasitology, Clinical center University of Sarajevo, Bosnia and Herzegovina
  • Amela Ljubović-Dedeić Institute of microbiology, immunnology and parasitology, Clinical center University of Sarajevo, Bosnia and Herzegovina

DOI:

https://doi.org/10.5644/Radovi.381

Keywords:

SRSVs, gastroentcritis, RT-PCR

Abstract

Small round-structured viruses (SRSVS) are causative agents of non-bacterial acute gastroenteritis at humans. They are members of family caliciviridac which are similar to picomaviruses in size (28-30 nm) and specific by cup-shaped outline on their surface. The virions contain single-stranded, positive-sense RNA (7.4 - 7,7 kb) and have not the envelope. Actually, SRSVs are members of caliciviridac NLVs genus (Norwalk like viruses). SRSV strains are: NV-8FIIa/68/US; KY-89/89/9; Sa-1283/84/J; SMA/76/US; 925/92/UK; OTH-25/89/9. The actual SRSVs oligonucleotide detection primers are signed as 36, 35, 69, 78 etc. and for which is known the 5' position. There are also oligonucleotide primers for which precise location within genome is not yet known (primer signed as 86,95,96 etc.). Obtained results presented in this paper showed high sensitivity and specifity of molecular SRSVs detection from stool specimens of clinical patients with specific clinical symptoms of acute gastroenteritis, by using of broadly reactive oligonucleotide primers (Ni and E3) in RT-PCR method of amplification and detection by transilluminator after separation in agarose gel electrophoresis.

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Published

13.08.2004

Issue

Vol. 33 (2004): Radovi

Section

Works

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Copyright (c) 2026 Đemo Subašić, Kemal Šerić, Amela Ljubović-Dedeić

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Using of Broadly Reactive Primer Pair in RT-PCR Detection of Small Round-Structured Viruses (SRSVS). (2004). Acta Medica Academica, 33, 85-92. https://doi.org/10.5644/Radovi.381

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