The Risk Assessment of Exposition to Heavy Metals on Cells of Chicken Embryo and Tissue Cultures in Ecotoxicology
DOI:
https://doi.org/10.5644/Radovi.300Abstract
The effects of metal ions — Ag+, Fe++, Sb++ on the hen embryo and tissue culture in final concentrations of 10-1 — 10-5 M was studied. Each concentration, soluted in 0,2 ml, was inoculated in the tissue of hen embryo, in amnion and in yolk sac as well as in tissue culture in vitro cells HeLa and GMK lines. Fe++, the essential element in the animal and in the human body and Ag+ and Sb++, which normally are not present as essential biological elements, manifested considerable differences in their toxical effects on the cell hen embryo as well as on the continual cell lines HeLa and GMK. It was observed stronger and faster toxicological effects of Ag+ and Sb++ than Fe++, which manifested their toxicological effects already first day after application regardless of the method of metal application. The death of embryo was evident very quickly after inoculation of metal ions into amnion and into the yolk sac. The toxically effects of Ag+ and Sb++ were faster manifested in the cell culture, where Ag+ and Sb++ caused the death of cells in the solution of 10-1 — 10-2 and 10-3 M, but Fe++ showed the same effects only in concentration of IO-1 M. It was observed that inoculation of those metal ions in amnion of the hen embryo caused a faster death of cells than the inoculation of the same metals in yolk sac.
References
Bothvvell, T. H., Finch, C. A.: Iron Metabolism, Little Brow. et Co Boston, 1962.
Browning, E.: Toxicity of Industrial Metals, Buttervvorth, London, 1969.
Conine, D. L., Yum, M. N., Martz, R. C. et al.: Toxicity of Sodium Pantafluorostannite, a New Anticariogenic Agent. I Comparisonof the Acute Toxicity of Sodiumpentafluorostannite, Sodium flouride and Stannous Chloride in Mice and or Rats. Toxicol. app. Pharmacol. 33, 21—26, 1975.
De Groot, A. P.: Subacute Toxacity of Inorganic Tin Influenced by Dietary Levels of Iron and Copper. Food Cosmet. Toxicol, 11, 955—962, 1973.
De Groot, A. P., Feron, V. J., Til, H. P.: Short-Terni Toxicity Studies on Home Salts and Oxides of Tin Rats. Food Cosmet. Toxicol. 11, 19—30, 1973.
Forschale-, I. et al.: Brit. J. Surg. 41, 379, 1954.
Hawkes, R. A.: General Principles Underlying Lab. Diagn. of Viral inf., ed. Lennette, E. H. and Schmidt, N. J. Diagan, Proc. for Viral, Ricketts, and Clamydial Inf., 1979.
Moeschlin, S. Schnider, U.: Schweiz, med. Wchr., 92, 1295, 1962.
Palmer, D. F., Dovvdle, W. R., Coleman, M. T. et al.: Advanced Lab. Techn. for Infl. Diagn., U. S. Depart. of Health, Education and Welfare Publi. Health Service 1975.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Marija Keser-Stanković, Danica Hlača, Dragan Stanković
This work is licensed under a Creative Commons Attribution 4.0 International License.
