New Aspects of Pharmacotherapy of Spinal Cord Contusionevaluation of the Protective Effects of D-Penicillamine and Cysteamine (MEA) on the Membrane Resting Potential of the Smooth Muscle Cells
DOI:
https://doi.org/10.5644/Radovi.265Abstract
In this work the protective effects of d-Penicillamine and cysteamine (MEA) on the membrane resting potential of smooth muscle cells after application of lead ions was studied. D-Penicillamine and MEA. like SH protectors on the cell membrane, after lead ions were added, showed different effects. D-Penicillamine causes strong initial membrane repolarisation of the smooth muscle cells of ileum (the decrease of membrane potential was from 42 mV to 15 mV).
Folowing application of lead ions the resting membrane potential was totally regenerated. Cysteamine at the start causes the decrease of the membrane resting potential (from 42,5 mV to 20 mV). During the course of the experiment the resting potential was gradually regenerated. Applied lead ions on cysteamine caused irreversible reduction of the membrane potential of smooth muscle cells Starting from the known facts that SH groups of enzymatic systems on the cell membrane are susceptible to the action of the heavy metals, particularly the lead, the application of those antidots which possess SH groups showed different effects. D-Penicillamine showed the protective effects on the cell membrane and cysteamine did non-inhibit the toxic effect of the lead.
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